serum osteocalcin (Novus Biologicals)
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Structured Review
Novus Biologicals
serum osteocalcin
Serum Osteocalcin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/serum osteocalcin/product/Novus Biologicals
Average 93 stars, based on 18 article reviews
Serum Osteocalcin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/serum osteocalcin/product/Novus Biologicals
Average 93 stars, based on 18 article reviews
serum osteocalcin - by Bioz Stars,
2026-02
93/100 stars
Images
1) Product Images from "Imbalanced Skeletal Muscle Mitochondrial Proteostasis Causes Bone Loss"
Article Title: Imbalanced Skeletal Muscle Mitochondrial Proteostasis Causes Bone Loss
Journal: Research
doi: 10.34133/research.0465
Figure Legend Snippet: Skeletal muscle-specific ablation of LONP1 causes bone loss and mechanical impairments. (A to D) Scheme (A) for proteomic analysis using muscle samples from HU and control mice. Data were obtained from published dataset PXD041190. (B) Heatmap showing differentially regulated proteins in HU muscles ( n = 5). (C) GO analysis from up-regulated proteins in HU muscles ( n = 5). (D) Bar graphs for comparison of normalized protein expression of LONP1 in muscle tissues from HU muscles and controls ( n = 5). (E to N) WT and LONP1 mKO male mice were harvested at 8 weeks old. (E and F) μCT pictures (E) and quantification (F) of trabecular bone parameters in the distal femur metaphysis ( n = 6). (G) Bending test analysis of elastic modulus, maximum force, compressive ultimate stress, and post-yield displacement of femurs ( n = 6). (H and I) μCT pictures (H) and quantification (I) of trabecular bone parameters in the fifth LVs ( n = 6). (J) Pictures of calcein double labeling in femur sections (scale bar, 50 μm) and quantification of MAR ( n = 5). (K) Images of ALP staining in femur sections (scale bar, 50 μm) and quantification of ALP + cell surface per bone surface (ALP + .s/BS) in femurs ( n = 5). (L) TRAP staining pictures in femur sections (scale bar, 50 μm) and quantification of TRAP + cell surface per bone surface (TRAP + .s/BS) in femurs ( n = 5). (M and N) Serum osteocalcin levels (M) and CTX-1 levels (N) ( n = 8). Data are shown as the mean ± SEM. * P < 0.05 versus corresponding controls. P values were determined by an unpaired 2-tailed Student’s t test.
Techniques Used: Control, Muscles, Comparison, Expressing, Labeling, Staining
Figure Legend Snippet: The sudden loss of LONP1 in mature muscles results in bone loss. (A) Diagrammatic drawing demonstrating the generation of tamoxifen-inducible muscle-specific deletion of Lonp1 (LONP1 HSA-MCM ). (B to I) LONP1 f/f and LONP1 HSA-MCM male mice were fed CD and injected by tamoxifen at 6 weeks old and harvested at 15 weeks old. (B) Representative quantification of LONP1 protein expression in skeletal muscles, eWAT, liver, and heart from LONP1 f/f and LONP1 HSA-MCM male mice. Quantification of the LONP1/β-actin ratio was determined by ImageJ ( n = 3). (C and D) μCT pictures (C) and quantification (D) of trabecular bone parameters in the distal femur metaphysis ( n = 5). (E) Bending test analysis of elastic modulus, maximum force, compressive ultimate stress, and post-yield displacement of femurs from LONP1 f/f and LONP1 HSA-MCM mice ( n = 5). (F and H) Pictures of ALP staining and TRAP staining in femur sections (scale bar, 50 μm). (G and I) Serum osteocalcin levels (G) and CTX-1 levels (I) of LONP1 f/f and LONP1 HSA-MCM mice ( n = 8). Data are shown as the mean ± SEM. * P < 0.05 versus corresponding controls. P values were determined by an unpaired 2-tailed Student’s t test. Ud, undetectable.
Techniques Used: Muscles, Injection, Expressing, Staining
Figure Legend Snippet: Skeletal muscle-specific overexpression of mitochondrial-retained ΔOTC protein induces bone loss. (A to J) NTG and MCK-ΔOTC male mice were harvested at 8 weeks old. (A and B) μCT pictures (A) and quantification (B) of trabecular bone parameters in the distal femur metaphysis ( n = 5). (C) Bending test analysis of elastic modulus, maximum force, compressive ultimate stress, and post-yield displacement of femurs from NTG and MCK-ΔOTC mKO. (D and E) μCT pictures (D) and quantification (E) of trabecular bone parameters in the fifth LVs ( n = 5). (F) Pictures of calcein double labeling in femur sections (scale bar, 50 μm) and quantitative analysis of MAR ( n = 5). (G) Images of ALP staining in femur sections (scale bar, 50 μm) and quantification of ALP + .s/BS ( n = 5). (H) Pictures of TRAP staining in femur sections (scale bar, 50 μm) and quantification of TRAP + .s/BS ( n = 5). (I and J) Serum osteocalcin levels (I) and CTX-1 levels (J) ( n = 8). Data are shown as the mean ± SEM. * P < 0.05 versus corresponding controls. P values were determined by an unpaired 2-tailed Student’s t test.
Techniques Used: Over Expression, Labeling, Staining
Figure Legend Snippet: Skeletal muscle mitochondrial proteostasis stress-induced bone loss is independent of ATF4. (A) Heatmap analysis of muscle-UPR mt myokines and Lonp1 or Atf4 genes in indicated mice (GSE192990; n = 2). (B to J) WT, LONP1 mKO, and LONP1/ATF4 DmKO male mice were harvested at 8 weeks old. (B and C) μCT pictures (B) and quantification (C) of trabecular bone parameters in the distal femur metaphysis ( n = 5 to 6). (D) Bending test analysis of elastic modulus, maximum force, compressive ultimate stress, and post-yield displacement of femurs ( n = 5). (E and F) μCT pictures (E) and quantification (F) of trabecular bone parameters in the fifth LVs ( n = 5 to 6). (G) Pictures of ALP staining in femur sections (scale bar, 50 μm) and quantification of ALP + .s/BS ( n = 5). (H) Pictures of TRAP staining in femur sections (scale bar, 50 μm) and quantification of TRAP + .s/BS ( n = 5). (I and J) Serum osteocalcin levels (I) and CTX-1 levels (J) ( n = 8). Data are shown as the mean ± SEM. * P < 0.05 versus corresponding controls. P values were determined by one-way ANOVA.
Techniques Used: Staining
